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Phase imaging is a powerful, dynamic force technique that can reveal many unique mechanical and chemical properties of a sample at the nanometer scale. In phase imaging (see Fig. F.9), an AFM cantilever is oscillated vertically near its mechanical resonance frequency while it is in close proximity to a sample. As the AFM tip comes in very close proximity to the sample surface, the amplitude of the cantilever s oscillation is reduced. The change in amplitude is measured and is used to track changes in the surface topography and roughness of the sample. Simultaneously, as the AFM tip encounters regions of di erent composition, a change in phase, relative to the phase of the drive signal, is measured and recorded. This change in phase is very sensitive to variations in material properties, including surface sti ness, elasticity, and adhesion. The phase shifts are measured and displayed in a very straightforward manner that facilitates quantitative analysis and interpretation. Both inorganic and organic materials have been examined with phase imaging. Phase imaging has been found to be particularly useful to map the various components of composite materials, to study variations in composition and contamination in materials, and to measure adhesion, surface hardness, and elasticity. It has been applied to thin- lm studies, the materials sciences, and composite characterization.

Click North Location to open the North Location dialog box and to set which direction is north. Setting the north location is important if you want to use the Sun Angle Calculator to create a distant light and if you want to create shadows. By default, north is the positive Y direction in the World Coordinate System (WCS). To change the default, type a new angle in the Angle box, use the slider bar, or click the clock face to specify the new angle. The positive Y axis is at 0 degrees, the positive X axis is at 90 degrees, and so on clockwise. If you ve saved a UCS, you can choose it from the list on the right side of the dialog box. AutoCAD uses the Y axis in that UCS as the default North direction.

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presses the N-terminal fragment ions so that only y-ions are observed, thus simplifying MALDI spectra. It is necessary to modify lysine residues (e.g. by guanidination) prior to sulphonation. A modi cation of this approach is to guanidinate N-termini in 2-D SDS PAGE gels that can later be tryptic digested and sulphonated. Conversion of lysine to homoarginine means trypsin does not cleave at these sites but this approach nonetheless allows sequencing by detection of y-ions by MALDI MS Phosphorous-containing reagents also introduce positive charge as phosphonium groups at the N-terminus using reagents such as phosphines and phosphonium salts (Figure 10.14). This causes preferential formation of N-terminal a- and b-ions which makes sequencing from MALDI fragmentation patterns possible (Figure 4.19).

A suspect word is one that the OCR Engine interprets differently from the closest match found in the Primary OCR Language Dictionary. The word is suspect because it may or may not be a correct interpretation. You might have proper names, industry terminology, abbreviations, and so on that the OCR Engine marks as suspects. Simply because the word(s) is marked as a suspect doesn t necessarily require changing the word. Therefore, when you review suspects, you have two choices: Either change the word to a correct spelling or inform Acrobat to leave it as is and move to the next suspect. After the Recognize Text dialog box disappears, the converted page(s) doesn t appear any different from before you began the OCR conversion. In order to see any words that may have been misinterpreted during the conversions, you need to access a menu command and tell Acrobat you want to view the suspect words. You have two choices in the OCR Text Recognition submenu.

In a similar manner, the correlation of the intensity with a sinusoidal signal produces hI2 x; y i I 00 x; y sin f x; y 14:170

28 VARIATIONS FROM THE TWYMAN GREEN CONFIGURATION Many variations of the Twyman Green interferometer have been invented; interesting among them are some small, compact interferometers designed by Van Heel and Simons (1967) and by Basile (1979) A carbon dioxide laser has been used as a light source of long wavelengths (1067m 1m) in a Twyman Green interferometer (Munnerlyn et al, 1969, Kwon et al, 1979, and Lewandowski et al, 1986) in order to measure unpolished or rough surfaces Another interesting development is the invention of a cheap interferometer whose defects are corrected by means of a hologram (Rogers, 1970) First, a photograph is taken in a very imperfect and inexpensive interferometer, introducing a large tilt This gives rise to an exceedingly ne set of fringes invisible to the naked eye but capable of being photographed.

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